| InputType: | PlantCellsorTissue |
| ResearchArea: | RNAMethylation |
| TargetApplication: | SampleIsolation |
| VesselFormat: | Columns/Tubes |
| 100%Guarantee: | 6months |
TheEpiQuik™ TotalRNAIsolationKit(Plant) providesafast,simple,andcost-effectivemethodforisolatingtotalRNAfromplanttissuesamples.DetergentsandchaotropicsaltareusedtolysecellsandinactivateRNase.Thespecializedhigh-saltbufferingsystemallowsRNAspecieslongerthan100basestobindtotheglassfibermatrixofthespincolumn.TheTotalRNAIsolationKitissuitableforavarietyofroutineapplicationsincludingRT-PCR,CDNASynthesis,NorthernBlotting,Differentialdisplay,PrimerExtensionandmRNAselection. Theentireprocedurecanbecompletedwithin60minutes. Thekithasthefollowingfeaturesandadvantages:
- Fastproceduredelivershigh-qualitytotalRNAin60minutes
- Ready-to-useRNAforhighperformanceinanydownstreamapplication
- ConsistentRNAyieldfromasmallamountofstartingmaterial
Principle&Procedure
ThiskitcontainsallthereagentsrequiredforsuccessfullyperformingRNAisolationdirectlyfromplanttissues.Afterlysis,binding,andwash,RNAiseasilyrecoveredinquantitiesofupto30µgusingspeciallydesignedcolumns.TotalRNAisthenreadytobeusedforavarietyofdownstreamapplications.
StartingMaterials
Theamountofstartingmaterialscanbe100mgoffreshplanttissueor25mgofdryplanttissue.Atotalof50standardextractionscanbeperformedwiththiskit. YieldofthetotalRNAcanbeupto30µg.Theyieldmayvarydependingonthesampletype.
RP(Pre-lysisBuffer)
W1(WashBuffer-1)
W2(WashBuffer-2)*
RE(ElutionBuffer)
P-Column
CollectionTubes
*Add30mlethanol(96~100%)totheW2(WashBuffer-2),andshakebeforeuse.
