TheEpiQuik™ TotalRNAIsolationKit(Tissue) providesafast,simple,andcost-effectivemethodforisolatingtotalRNAfromtissuesamples.DetergentsandchaotropicsaltareusedtolysecellsandinactivateRNase.Thespecializedhigh-saltbufferingsystemallowsRNAspeciesbasestobindtotheglassfibermatrixofthespincolumnwhilecontaminantspassthroughthecolumn.Impuritiesareefficientlywashedaway,andthepureRNAiselutedwithREBufferwithoutphenolextractionoralcoholprecipitation.RNApurifiedwithTheTotalRNAIsolationKit(Tissue)issuitableforavarietyofroutineapplicationsincludingRT-PCR,CDNASynthesis,NorthernBlotting,Differentialdisplay,PrimerExtensionandmRNASelection.Theentireprocedurecanbecompletedwithin25-40minutes. Thekithasthefollowingfeaturesandadvantages:

• Fastproceduredelivershigh-qualitytotalRNAin25-40minutes
• Ready-to-useRNAforhighperformanceinanydownstreamapplication
• ConsistentRNAyieldfromasmallamountofstartingmaterial

Principle&Procedure
ThiskitcontainsallthereagentsrequiredforsuccessfullyperformingRNAisolationdirectlyfromtissue.Afterlysis,binding,andwash,RNAiseasilyrecoveredinquantitiesofupto30µgusingspeciallydesignedcolumns.TotalRNAisthenreadytobeusedforavarietyofdownstreamapplications.

StartingMaterials
Theamountofstartingmaterialscanbeupto30mgoftissue,orupto25mgofparaffin-embeddedtissue.Atotalof50standardextractionscanbeperformedwiththiskit. YieldofthetotalRNAcanbeupto30µg.Theyieldmayvarydependingonthesampletype.

RR(LysisBuffer)
W1(WashBuffer-1)
W2(WashBuffer-2)*
RE(ElutionBuffer)
T-Column
CollectionTubes

*Add30mlethanol(96~100%)totheW2(WashBuffer-2),andshakebeforeuse.