TheEpiQuik™ TotalRNAIsolationKit providesafast,simple,andcost-effectivemethodforisolatingtotalRNAfromwholeblood,mammaliancells,bacterialcells,andfungalcells.DetergentsandchaotropicsaltareusedtolysecellsandinactivateRNase.Thespecializedhigh-saltbufferingsystemallowsRNAspeciesbasestobindtotheglassfibermatrixofthespincolumnwhilecontaminantspassthroughthecolumn.Impuritiesareefficientlywashedaway,andthepureRNAiselutedwiththeREBufferwithoutphenolextractionoralcoholprecipitation.TheRNApurifiedwiththeTotalRNAIsolationKitissuitableforavarietyofroutineapplications,includingtheRT-PCR,CDNASynthesis,NorthernBlotting,Differentialdisplay,PrimerExtensionandmRNAselection.Theentireprocedurecanbecompletedwithin25-40minutes. Thekithasthefollowingfeaturesandadvantages:

• Fastproceduredelivershigh-qualitytotalRNAin 25-40minutes
• Ready-to-useRNAforhighperformanceinanydownstreamapplication
• ConsistentRNAyieldfromasmallamountofstartingmaterial

Principle&Procedure
ThiskitcontainsallthereagentsrequiredforsuccessfullyperformingRNAisolationdirectlyfromblood,culturedcells,orfungus.Afterlysis,binding,andwash,RNAiseasilyrecoveredinquantitiesofupto30µgusingspeciallydesignedcolumns.TotalRNAisthenreadytobeusedforavarietyofdownstreamapplications.

StartingMaterials
Theamountofstartingmaterialscanbeupto300µlofwholeblood,10^7mammaliancells,10^9bacterialcells,and10^8fungalcells.Atotalof50standardextractionscanbeperformedwiththiskit. YieldofthetotalRNAcanbeupto30µg.Theyieldmayvarydependingonthesampletype.

RL(Pre-lysisBuffer)
RA(LysisBuffer)
RO(Pre-lysisBuffer-Bacteria/Fungus)
W1(WashBuffer-1)
W2(WashBuffer-2)*
RE(ElutionBuffer)
R-Column
CollectionTubes

*Add30mlethanol(96~100%)totheW2(WashBuffer-2),andshakebeforeuse.