TheEpiNext™ DNA PurificationHTSystem(alsoknownas"EpiNext™beads"or"MQ beads") utilizesmagneticbeadtechnologyforhighthroughputDNAorPCRampliconpurificationandDNAsizeselection.Thesystemcanalsobe usedforconcentratingDNAandissuitableforselectivelyandconsistentlycapturingDNAfragmentsorPCRampliconsthatare100bpsorlargerinsize.Thesystemhasthefollowingadvantages: - Optimizedbufferchemistries: CompleteseparationofDNAorPCRamplicons.ItcanalsobeusedforDNAsizeselectionbasedontheratioofbeadstoDNAsamplevolume.
- Fastandstraightforward:Theentireprocedurecanbefinishedinjust30minfor96samplesandishighlyamenabletoavarietyofautomationplatforms.Nogels,filtration,centrifugation,or columns areneeded.
- Efficientclean-up:Removalofexcessprimers,adaptors,nucleotides,salts,enzymes,andPCRinhibitingsubstancessuchaspolysaccharides,polyphenols,lipidsanddyes.
- HighrecoveryofDNA:Higherthan98%recoveryofinputDNA.
- Manualandautomationfriendly:Scalableforuseinsingletubeor96-wellplateformats.
- Ultimatevalue:SuperiorperformanceforlessthanhalfthepricevsAgencourt® AMPure® XPbeads,without havingtochangeanyofyourexistingworkflowsorprotocols.
BackgroundInformation
ObtaininghighrecoveryofpurifiedDNAorselectedDNAfragmentsiscriticalfordownstreamapplicationsthatuseDNAsamplesincludingPCR,sequencing,cloning,microarray,andDNAfragmentanalysis,regardlessoftheplatformused. Principle&Procedure
ThesystemcontainsanoptimizedMQbindingbeadsolutiondevelopedbyEpigentekwhichallowsDNAorPCRampliconstobindtightlytothebeads.Excessprimers,adaptors,nucleotides,salts,enzymes,andPCRinhibitingsubstancescanberemovedbysimplywashingthebeads.OptimizationofMQbeadratiotoinputDNAallowsDNAsizeselectionbytheremovaloflargerorsmallerDNAfragmentsandrecoveryofdesiredtargetsizeDNAfragments. StartingMaterials
DNAfragmentsofvariouslengths.Inputamountcanbefrom0.1ngto1µg.Thebeadvolumesprovidedinthesystemof4mlor8mlpermita totalof96or192standardpurifications,respectively(use0.5µgofDNAin20µlofsolution);or48or96standardDNAsizeselections,respectively(use50µlofinputDNAsolution). Agencourt® AMPure®isaregisteredtrademarkofBeckmanCoulter,Inc. | 
 Fig.1. WorkflowoftheEpiNext™DNAPurificationHTSystem.
Fig.2. Gelpictureafterpurificationof800ngofDNAMarker(Hyperladder50bpladder,Bioline)usingtheEpiNext™DNAPurificationHTSystemwithvaryingratiosofbeadsfrom0.9Xto2.1X.AMPureXPbeadswereusedasthecontrol.M:MarkersizeofAgilentDNAchipkit. 
Fig.3. PercentageofrecoveryofinputDNAfragmentsby"AMPureXP"beadsvs“EpiNext”beads:780ngofDNAmarker(Hyperladder50bpladder,Bioline)werepurifiedusingtheEpiNext™DNAPurificationHTSystemat1.8X.AgencourtAMPureXPbeads(1.8X)wereusedasthecontrol.Recoveryyieldof100bporhigherDNAfragmentswasquantifiedwithanAgilentBioanalyzer2100. |
