TheEpiQuik™One-StepDNAHydrolysisKitisacompletesetofessentialcomponentswhichenablestheexperimentertorapidlyhydrolyzeDNAtodeoxynucleosideswithaprocessthatcanbeperformedinasingleincubation.IncomparisontocurrentlyusedmethodsfordigestingDNAwithtri-enzymes,whichhaveseveraldrawbacksandexcessivetimeconsumption,theEpiQuik™One-StepDNAHydrolysisKitprovidesahighlyefficientenzymatichydrolysisinminimaltime.ItisalsoverysuitablefordigestingDNAfromvariousBIOLOGicalmaterials. 

WHYCHOOSETHEEPIQUIK™ONE-STEPDNAHYDROLYSISKIT,

• Afastone-stepprocedure,whichcanbefinishedinasshortas1hour.
• Performedinasingleincubation,withouttheneedforDNAdenaturation.
• Highlyefficientenzymatichydrolysis:non-specificallydigestsDNAoroligonucleotidesintosinglenucleosides.
• 96-wellmicroplateformatmakesthesamplepreparationflexIBLeforusewithhighthroughputformats.

Fig.1. TheEpiQuik™One-StepDNAHydrolysisKitsimplyappliesourproprietaryenzymaticDNAdigestionsolutiontoDNAoroligonucleotides.AftertreatmentwiththeDNAdigestionbuffer,DNAiseasilydigestedintosinglenucleosideswithoutphosphategroups.

Fig.2. 0.5µgand2µgoffullymethylatedHeLaDNAweretreatedwiththekitat37Cfor1handthenanalyzedbygelelectrophoresis.1:2ugofundigestedDNA;2:0.5ugofdigestedDNA;3:2ugofdigestedDNA;4:1ugofdeoxynucleoside;“M”isa2kbDNAladder. 

Fig.3. 1ugoffullymethylatedHeLaDNAwasdigestedwiththekitat37Cfro1and2h,respectively. ThedigestedDNAwasfluorescentlyquantified. 1:Blank;2:Deoxynucleosidecontrol;3:undigestedDNA;4:undigestedtri-oligos(3nt);5:undigestedpenta-oligos(5nt);6: DNAdigestedfor1h;7:DNAdigestedfor2h.