The FitAmp™GelDNAIsolationKit isacompletesetofessentialcomponentswhichenablestheexperimentertoefficientlyisolateDNAfromlowmeltingpointagarosegel.Theentireprocedurecanbecompletedwithin15minutesandissuitablefordirectuseinthefollowingapplications:ligation/transformation,sequencing,restrictiondigestion,microarrayanalysis,labeling,microinjection,and invitro transcription. 

WHYCHOOSETHEFITAMP™GELDNAISOLATIONKIT,

• Fastprocedure(<15minutes).
• Recoveryofultra-pureDNAfromagarosegelathighconcentrationinminimalvolume.
• Useofproprietarybindingsolutionwithoutchaotropicorotherirritantreagents.

Fig.1. TheFitAmp™GelDNAIsolationKitappliesourproprietarylysisandbindingbuffertoagel-DNAsample.AftertransferringthesampletothespecificallydesignedF-SpinColumnandwashwiththewashingbuffer,theDNAiseasilyrecoveredin8-20µlwithelutionsolution.IsolatedDNAisreadyfordownstreamapplication. 

 
Fig.2. DifferentsizesofDNAfragmentsinlanes2to5weresuccessfullyrecoveredfromagaroseusingthe FitAmp™GelDNAIsolationKit.