The TuMinute™PCRClean-UpKit isacompletesetofessentialcomponentswhichenablestheexperimentertoefficientlycleanupPCRproductsfromvarioussourcesincludingconventionalorrealtimethermalcyclingPCRandisothermalgeneamplification(PCR).Theentireprocedurecanbecompletedwithin2minutesandissuitablefordirectuseinthefollowingapplications:ligation/transformation,sequencing,restrictiondigestion,microarrayanalysis,labeling,microinjection,and invitro transcription. 

WHYCHOOSETHETUMINUTE™PCRCLEAN-UPKIT,

• Extremelyfast2minutecleanupprocedure.
• Ultra-purityandrecoveryofsmallPCRproductswithefficientremovalofcontaminants(salts,proteins,enzymes,nucleotidesandprimers).
• Useofpreparatorybindingsolutionandnonchaotropicorotherirritantreagents.

Fig.1. The TuMinute™PCRClean-UpKit simplyappliesourproprietarybindingbuffertoPCRproducts.AftertransferringthesampletothespeciallydesignedF-SpinColumnandwashingwithwashingbuffer,thePCRproductsareeasilyrecoveredin8-20µlelutionbuffer.PurifiedPCRproductsarereadyfordownstreamapplication.