TheEpiQuik™NuclearExtractionKitII(NucleicAcid-Free)isacompletesetofoptimizedreagentstoprovideasimpleandselectivemethodforisolatingnuclearproteinsfreeofnucleicacidsattached,tobeusedinavarietyofapplicationsinjust60minutes.TheseapplicationsmayincludeWesternblot,protein-DNAbindingassays,nuclearenzymeassays,andanyotherproceduresrequiringoptimizednucleicacid-freenuclearproteinsbutnotenzymaticactivity.Theprotocolisfastandeasy-to-use,andalsoisolatesveryabundantyieldsofnuclearextractfrommammaliancellsortissuesamples.Someofthekit"shighlightedfeaturedincludethefollowing:

• Pre-optimizedandsimplestep-wise1hourprotocol.
• Convenientlyincludesallessentialreagentstocarryoutanucleicacid-freenuclearextraction.
• StandardizedprocedureforreproducIBLeresults.

Principle&Procedure
TheEpiQuik™NuclearExtractionKitII(NucleicAcid-Free)simplyappliesourproprietarynuclearproteinisolationbufferstocell/tissues.Aftertreatmentwithpre-lysisandlysisbuffers,thenucleicacid-freenuclearproteinsareeasilyextractedforimmediateuseorstorageatproperconditions.

Note:Thisitemwasformerlycatalogno.OP-0002-2(OP00022,OP-00022,OP0002-2)

 
Fig.1.SchematicimageoftheEpiQuik™NuclearExtractionKitII(NucleicAcid-Free).