The EpiQuik™NuclearExtractionKitisacompletesetofoptimizedreagentstoprovideasimpleandselectivemethodforisolatingnuclearproteinsusedforavarietyofapplicationsinjust60minutes.TheseapplicationsmayincludeWesternblot,protein-DNAbindingassays,nuclearenzymeassays,andanyotherproceduresrequiringoptimizednuclearproteins.Theprotocolisfastandeasy-to-use,andalsoisolatesveryabundantyieldsofnuclearextractfrommammaliancellsortissuesamples. ThekitisalsospecificallydesignedtomeettherequirementsofnuclearextractsusedinvariousotherepigeneticproductsfromEpigentek,including DNMT, HDAC,and HAT assays.Someofthekit"shighlightedfeaturesincludethefollowing:

• Pre-optimizedandsimplestep-wise1hourprotocol.
• Convenientlyincludesallessentialreagentstocarryoutanuclearextraction.
• StandardizedprocedureforreproducIBLeresults.

Principle&Procedure
The EpiQuik™NuclearExtractionKitsimplyappliesourproprietarynuclearproteinisolationbufferstocell/tissues.Aftertreatmentwithpre-lysisandlysisbuffers,thenuclearproteinsareeasilyextractedforimmediateuseorstorageatproperconditions.

StartingMaterials&Yield
Atotalof100standardextractions(using10^7 cellsor20mgtissues)canbeisolatedfrom mammaliancellsortissuesampleswiththiskit.Totalyieldcanbeupto100µgperoptimalextraction,althoughthe resultsmaysomewhatvarydependingonthecellortissuetype.

NE1(10XPre-ExtractionBuffer)
NE2(ExtractionBuffer)
PIC(1000XProteaseInhibitorCocktail)
1000XDTTSolution
UserGuide