Background
Modulationofchromatinstructureplaysanimportantroleintheregulationoftranscriptionineukaryotes.Thenucleosome,madeupofDNAwoundaroundeightcorehistoneproteins(twoeachofH2A,H2B,H3,andH4),istheprimarybuildingblockofchromatin.Theamino-terminaltailsofcorehistonesundergovariouspost-translationalmodifications,includingacetylation,phosphorylation,methylation,andubiquitination.Thesemodificationsoccurinresponsetovariousstimuliandhaveadirecteffectontheaccessibilityofchromatintotranscriptionfactorsand,therefore,geneexpression.Inmostspecies,histoneH2BisprimarilyacetylatedatLys5,12,15,and20.HistoneH3isprimarilyacetylatedatLys9,14,18,23,27,and56.AcetylationofH3atLys9appearstohaveadominantroleinhistonedepositionandchromatinassemblyinsomeorganisms.PhosphorylationatSer10,Ser28,andThr11ofhistoneH3istightlycorrelatedwithchromosomecondensationduringbothmitosisandmeiosis.PhosphorylationatThr3ofhistoneH3ishighlyconservedamongmanyspeciesandiscatalyzedbythekinasehaspin.Immunostainingwithphospho-specificantibodiesinmammaliancellsrevealsmitoticphosphorylationatThr3ofH3inprophaseanditsdephosphorylationduringanaphase.
Description
Rabbitpolyclonal antibodytoHistoneH3R17SymmetricDimethyl. Formulation
PBSwith0.02%sodiumazide,50%glycerol,pH7.3.
Immunogen
AsyntheticmethylatedpeptidecorrespondingtoresiduessurroundingArg17ofhumanhistoneH3.
Specificity
Human,Mouse,Rat,Predictedtoreactwithawiderangeofspecies
Isotype
IgG Purification
Affinitypurified.
Storage
Storeat-20°Cforshortterm.Aliquotandstoreat-80°Cforlongterm.Avoidmultiplefreeze/thawcycles. AlternativeNames
H3R17me2s,HIST1H3J,H3/j,H3FJ,HistoneH3.1,HistoneH3/a,HistoneH3/b,HistoneH3/c,HistoneH3/d,HistoneH3/f,HistoneH3/h,HistoneH3/I,HistoneH3/j,HistoneH3/k,HistoneH3/l,H3Arginine17me2s, H3R17m2s Application
WB(1:500-1:2000),IHC(1:50-1:200),IF(1:50-1:200)
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 Fig.1. WesternblotanalysisofextractsofHeLacelllineandH3proteinexpressedinE.coli.,usingHistoneH3R17SymmetricDimethylPolyclonalAntibody.
Fig.2. Dot-blotanalysisofallsortsofmethylationpeptidesusingHistoneH3R17SymmetricDimethylPolyclonalAntibody. 
Fig.3. Immunofluorescenceanalysisof293TcellusingHistoneH3R17SymmetricDimethylPolyclonalAntibody.Blue:DAPIfornuclearstaining. |
